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By tagging the substrates with ubiquitin, E3 ligases provide a platform for protein-protein interactions that can modulate the activity, localization or interaction of the target proteins, thereby triggering different biological signals to regulate a variety of cellular processes including cell cycle, cell division, stress response, DNA repair and apoptosis etc 1,2,3,4

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In this circumstance, applying heat would cause more swelling and pain, so the usual treatment for the first few days after an MCL tear is to ice it down

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